CRYO ARMTM 300 II

La cryo-microscopie est une technique utilisée pour l’observation des molécules cellulaires et biologiques à des températures cryogéniques. Le CRYO ARM^TM 300 II intègre les dernières technologies JEOL pour la cryo-microscopie : Cold FEG, filtre en énergie Omega, système d’échange automatique des échantillons à température cryogénique…

Le système permet l’échange d’un ou de plusieurs échantillons rendant les études cryo plus flexibles. De plus, la combinaison du canon Cold FEG, avec le filtre Omega et les Hole Free Phase Plate améliore grandement le contraste des images acquises sur des échantillons biologiques. Le développement de cette dernière génération de MET incorpore de nombreux automatismes pour faciliter son utilisation et l’acquisition des données en particule isolée et cryo-tomographie.

Collection ultra rapide de données

Le CRYO ARM ™ 300 II permet une acquisition de données à très grande vitesse en combinant le déplacement du faisceau avec un mouvement précis de la platine goniométrique. De plus, l'excellent contrôle du faisceau de ce système minimise l’aberration de coma.
Par conséquent, le vitesse d’acquisition peut être augmentée de façon importante (> 2200 films / heures) sans dégrader la qualité d'image.

Illumination, configuration example en mode standard et mode Koehler (CRYOARM300)

Mode sans Frange de Fresnel

En plus de l’illumination conventionnelle, le CRYO ARM ™ 300 II dispose d'une illumination unique en « mode Koehler ». Ce mode permet de s’affranchir des franges d'interférence et élimine les dommages causés par le faisceau d'électrons aux zones non utilisées pour l'imagerie. Il vous permet d'obtenir plus d'images.

Bibliographie

voir les références

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